Region Of Interest

You may define multiple Regions of Interest (ROI) to allow you to more easily compare overlaid AFM and optical images and produce publication-ready images.

Create a Region of Interest

	Using the ruler tool, draw a box on the MIRO canvas.
	Click the Create a Region of Interest View icon to open an ROI window that displays up to 8 layers in that box plus the overlaid image.
	The number of layers in the ROI is controlled by the Layer Limit parameter in the Configure ROI View Settings window:

The layers displayed in an ROI view are controlled by selecting the Show/Hide button in the MIRO Tree tab.

You may open up to 5 ROI views. The active ROI will be displayed in the MIRO Canvas window as a solid yellow box while the inactive ROIs will be shown as dashed boxes.

The default ROI window is 2 layers plus the overlaid image:

NOTE: Regions of interest are square. If you draw a rectangle, the narrowest side will be used to define the square.

Configure the Region of Interest

Drag a corner or an edge of the ROI window to resize and/or reformat it. The ROI window may be rectangular (square if the total number of images is a power of 2), horizontal or vertical. Resize the window and click in a blank area to easily eliminate that portion.

Configure the ROI window by either clicking the Configure the ROI icon, or right-click in the ROI title bar and select Configure View. This opens the Configure ROI View Settings window.

Configure ROI View Controls:

Auto Update On Canvas Change: Automatically updates the ROI view when a change is made to the MIRO Canvas, e.g. showing or hiding a layer. Unchecking this box will freeze the ROI view. You may want to use this feature if you are changing the MIRO canvas but want to keep the ROI view from changing. You may manually refresh the ROI view by either clicking the Refresh the ROI icon or by right-clicking in the ROI title bar and clicking Refresh/Update Images.

Show Overlaid/Blended Image: Displays, as the final panel, the overlaid and blended image as shown in the MIRO Canvas window.
Show Scale and Color Bar: Shows the layer label, ROI dimensions, and vertical scale:

Show All RGB in Layer Image: Shows Red (R), Green (G) and Blue (B) regardless of the settings in the Color tab.
Image Cursor Type: May be a Cross, Line or Box (default). The same cursor will appear in all images in that ROI window.
Background Color: The intensity of the areas of the ROI that have no image with 0 being the darkest and 255 the lightest.
Layer Limit: The number of layers displayed is set in the Layer Limit box. You may display up to 8 layers in an ROI window.

Click OK to accept your changes or Cancel to return to the unchanged ROI window.

Regions of interest are saved when you save MIRO (*.iro or *.xml) files. The Regions of Interest will open when you open the saved MIRO file.

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